[HTML][HTML] Dominant negative forms of Akt (protein kinase B) and atypical protein kinase Cλ do not prevent insulin inhibition of phosphoenolpyruvate carboxykinase …
K Kotani, W Ogawa, Y Hino, T Kitamura, H Ueno… - Journal of Biological …, 1999 - ASBMB
Transcriptional regulation of phosphoenolpyruvate carboxykinase (PEPCK), the rate-limiting
enzyme in hepatic gluconeogenesis, by insulin was investigated with the use of adenovirus
vectors encoding various mutant signaling proteins. Insulin inhibited transcription induced
by dexamethasone and cAMP of a chloramphenicol acetyltransferase (CAT) reporter gene
fused with the PEPCK promoter sequence in HL1C cells stably transfected with this
construct. A dominant negative mutant of phosphoinositide (PI) 3-kinase blocked insulin …
enzyme in hepatic gluconeogenesis, by insulin was investigated with the use of adenovirus
vectors encoding various mutant signaling proteins. Insulin inhibited transcription induced
by dexamethasone and cAMP of a chloramphenicol acetyltransferase (CAT) reporter gene
fused with the PEPCK promoter sequence in HL1C cells stably transfected with this
construct. A dominant negative mutant of phosphoinositide (PI) 3-kinase blocked insulin …