[HTML][HTML] Engineering a glucose-responsive human insulin-secreting cell line from islets of Langerhans isolated from a patient with persistent hyperinsulinemic …
WM MacFarlane, JC Chapman, RM Shepherd… - Journal of Biological …, 1999 - ASBMB
Persistent hyperinsulinemic hypoglycemia of infancy (PHHI) is a neonatal disease
characterized by dysregulation of insulin secretion accompanied by profound hypoglycemia.
We have discovered that islet cells, isolated from the pancreas of a PHHI patient, proliferate
in culture while maintaining a beta cell-like phenotype. The PHHI-derived cell line (NES2Y)
exhibits insulin secretory characteristics typical of islet cells derived from these patients, ie
they have no K ATP channel activity and as a consequence secrete insulin at constitutively …
characterized by dysregulation of insulin secretion accompanied by profound hypoglycemia.
We have discovered that islet cells, isolated from the pancreas of a PHHI patient, proliferate
in culture while maintaining a beta cell-like phenotype. The PHHI-derived cell line (NES2Y)
exhibits insulin secretory characteristics typical of islet cells derived from these patients, ie
they have no K ATP channel activity and as a consequence secrete insulin at constitutively …