TCR, LFA-1, and CD28 play unique and complementary roles in signaling T cell cytoskeletal reorganization
CE Sedwick, MM Morgan, L Jusino… - The Journal of …, 1999 - journals.aai.org
CE Sedwick, MM Morgan, L Jusino, JL Cannon, J Miller, JK Burkhardt
The Journal of Immunology, 1999•journals.aai.orgT cells interacting with APCs undergo rearrangement of surface receptors and cytoskeletal
elements to face the zone of contact with the APC. This polarization process is thought to
affect T cell signaling by organizing a specialized domain on the T cell surface and to direct
T cell effector function toward the appropriate APC. We have investigated the contribution of
TCR, CD28, and LFA-1 signaling to T cell cytoskeletal polarization by assaying the response
of an Ag-specific Th1 clone toward a panel of transfected APCs expressing MHC class II …
elements to face the zone of contact with the APC. This polarization process is thought to
affect T cell signaling by organizing a specialized domain on the T cell surface and to direct
T cell effector function toward the appropriate APC. We have investigated the contribution of
TCR, CD28, and LFA-1 signaling to T cell cytoskeletal polarization by assaying the response
of an Ag-specific Th1 clone toward a panel of transfected APCs expressing MHC class II …
Abstract
T cells interacting with APCs undergo rearrangement of surface receptors and cytoskeletal elements to face the zone of contact with the APC. This polarization process is thought to affect T cell signaling by organizing a specialized domain on the T cell surface and to direct T cell effector function toward the appropriate APC. We have investigated the contribution of TCR, CD28, and LFA-1 signaling to T cell cytoskeletal polarization by assaying the response of an Ag-specific Th1 clone toward a panel of transfected APCs expressing MHC class II alone or in combination with ICAM-1 or B7-1. We show that polarization of talin, an actin-binding protein, occurs in response to integrin engagement. In contrast, reorientation of the T cell microtubule-organizing center (MTOC) is dependent on and directed toward the site of TCR signaling, regardless of whether integrins or costimulatory molecules are engaged. MTOC reorientation in response to peptide-MHC complexes is sensitive to the phosphatidylinositol 3-kinase inhibitor wortmannin. CD28 coengagement overcomes this sensitivity, as does activation via Ab cross-linking of the TCR or via covalent peptide-MHC complexes, suggesting that phosphatidylinositol 3-kinase is not required per se but rather plays a role in signal amplification. Engagement of TCR in trans with LFA-1 results in separation of MTOC reorientation and cortical cytoskeletal polarization events, indicating that the two processes are not directly mechanistically linked. These studies show that T cells mobilize individual cytoskeletal components in response to distinct and specific cell surface interactions.
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