[HTML][HTML] Cell density and growth-dependent down-regulation of both intracellular calcium responses to agonist stimuli and expression of smooth-surfaced …

T Koizumi, H Hikiji, WS Shin, T Takato, S Fukuda… - Journal of Biological …, 2003 - ASBMB
A two-dimensional intracellular Ca 2+([Ca 2+] i) imaging system was used to examine the
relationship between [Ca 2+] i handling and the proliferation of MC3T3-E1 osteoblast-like
cells. The resting [Ca 2+] i level in densely cultured cells was 1.5 times higher than the [Ca
2+] i level in sparsely cultured cells or in other cell types (mouse fibroblasts, rat vascular
smooth muscle cells, and bovine endothelial cells). A high resting [Ca 2+] i level may be
specific for MC3T3-E1 cells. MC3T3-E1 cells were stimulated with ATP (10 μm), caffeine (10 …