[HTML][HTML] Interactions of fibrillin-1 with heparin/heparan sulfate, implications for microfibrillar assembly
K Tiedemann, B Bätge, PK Muller… - Journal of Biological …, 2001 - ASBMB
Fibrillin-1 is a major constituent of the 10–12 nm extracellular microfibrils. Here we identify,
characterize, and localize heparin/heparan sulfate-binding sites in fibrillin-1 and report on
the role of such glycosaminoglycans in the assembly of fibrillin-1. By using different binding
assays, we localize two calcium-independent heparin-binding sites to the N-terminal (Arg 45–
Thr 450) and C-terminal (Asp 1528–Arg 2731) domains of fibrillin-1. A calcium-dependent-
binding site was localized to the central (Asp 1028–Thr 1486) region of fibrillin-1. Heparin …
characterize, and localize heparin/heparan sulfate-binding sites in fibrillin-1 and report on
the role of such glycosaminoglycans in the assembly of fibrillin-1. By using different binding
assays, we localize two calcium-independent heparin-binding sites to the N-terminal (Arg 45–
Thr 450) and C-terminal (Asp 1528–Arg 2731) domains of fibrillin-1. A calcium-dependent-
binding site was localized to the central (Asp 1028–Thr 1486) region of fibrillin-1. Heparin …