The role of apolipoprotein A-IV in reverse cholesterol transport studied with cultured cells and liposomes derived from an ether analog of phosphatidylcholine
O Stein, Y Stein, M Lefevre, PS Roheim - Biochimica et Biophysica Acta …, 1986 - Elsevier
Cholesterol efflux was studied in a model system in culture using apolipoproteins and
phospholipids added in the form of liposomes at concentrations expected to be present in
the extracellular fluid. Fibroblasts were seeded in medium containing [3 H] cholesterol-
labeled serum, grown till confluent, and the [3 H] cholesterol efflux was studied in serum-free
medium. Addition of delipidated HDL apolipoprotein resulted in a very low release of [3 H]
cholesterol, which did not increase with time of exposure or concentration of …
phospholipids added in the form of liposomes at concentrations expected to be present in
the extracellular fluid. Fibroblasts were seeded in medium containing [3 H] cholesterol-
labeled serum, grown till confluent, and the [3 H] cholesterol efflux was studied in serum-free
medium. Addition of delipidated HDL apolipoprotein resulted in a very low release of [3 H]
cholesterol, which did not increase with time of exposure or concentration of …