Quantitative measurement of gastrointestinal blood loss: II. Determination of 24-hour fecal blood loss by a chemical photospectrometric technique
FG Ebaugh, WL Beeken - The Journal of laboratory and clinical …, 1959 - translationalres.com
FG Ebaugh, WL Beeken
The Journal of laboratory and clinical medicine, 1959•translationalres.com1. A simple and reasonably precise chemical method employing the benzidine base-H 2 O 2
reaction for measuring 24-hour fecal hemoglobin excretion has been described. 2.2. Forty-
nine men were found to shed 1.9±1.3 ml.(1 standard deviation) of whole blood per day in the
feces and 22 women 1.2±1.0 ml. per day. Men showed a significantly greater excretion of
blood in the feces than women (P< 0.02). 3.3. The quantitative benzidine test resulted in a
12 to 41 per cent higher apparent fecal blood loss as measured by the quantitative …
reaction for measuring 24-hour fecal hemoglobin excretion has been described. 2.2. Forty-
nine men were found to shed 1.9±1.3 ml.(1 standard deviation) of whole blood per day in the
feces and 22 women 1.2±1.0 ml. per day. Men showed a significantly greater excretion of
blood in the feces than women (P< 0.02). 3.3. The quantitative benzidine test resulted in a
12 to 41 per cent higher apparent fecal blood loss as measured by the quantitative …
Abstract
- 1.1. A simple and reasonably precise chemical method employing the benzidine base-H2O2 reaction for measuring 24-hour fecal hemoglobin excretion has been described.
- 2.2. Forty-nine men were found to shed 1.9 ± 1.3 ml. (1 standard deviation) of whole blood per day in the feces and 22 women 1.2 ± 1.0 ml. per day. Men showed a significantly greater excretion of blood in the feces than women (P < 0.02).
- 3.3. The quantitative benzidine test resulted in a 12 to 41 per cent higher apparent fecal blood loss as measured by the quantitative benzidine test.
- 4.4. Oral ferrous sulfate caused a significant increase (about 1 ml.) in apparent fecal blood loss as measured by the quantitative benzidine test.
- 5.5. All the data in this study was obtained on subjects on ad libitum diets. It would appear feasible to employ this test as a screening procedure without imposing dietary restrictions.
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