Experimental vaccine activities of recombinant E1 and E2 glycoproteins and hypervariable region 1 peptides of hepatitis C virus in chimpanzees
M Esumi, T Rikihisa, S Nishimura, J Goto, K Mizuno… - Archives of …, 1999 - Springer
M Esumi, T Rikihisa, S Nishimura, J Goto, K Mizuno, YH Zhou, T Shikata
Archives of virology, 1999•SpringerA chimpanzee was immunized with two recombinant envelope glycoproteins E1 and E2 of
hepatitis C virus (HCV), strain HCV-N2, and the hypervariable region 1 (HVR1) peptides of a
different isolate, HCV-# 6, then received an intravenous inoculation of 10 chimpanzee
infectious doses of HCV-# 6. With high humoral immune response against E1 and E2 but a
low response against HVR1, the vaccinee became infected with the HCV. However, after
increasing the titer of anti-HVR1 against HCV-# 6, the vaccinee showed protection …
hepatitis C virus (HCV), strain HCV-N2, and the hypervariable region 1 (HVR1) peptides of a
different isolate, HCV-# 6, then received an intravenous inoculation of 10 chimpanzee
infectious doses of HCV-# 6. With high humoral immune response against E1 and E2 but a
low response against HVR1, the vaccinee became infected with the HCV. However, after
increasing the titer of anti-HVR1 against HCV-# 6, the vaccinee showed protection …
Summary
A chimpanzee was immunized with two recombinant envelope glycoproteins E1 and E2 of hepatitis C virus (HCV), strain HCV-N2, and the hypervariable region 1 (HVR1) peptides of a different isolate, HCV-#6, then received an intravenous inoculation of 10 chimpanzee infectious doses of HCV-#6. With high humoral immune response against E1 and E2 but a low response against HVR1, the vaccinee became infected with the HCV. However, after increasing the titer of anti-HVR1 against HCV-#6, the vaccinee showed protection. Neutralization of HCV-#6 with the antiserum from this protected vaccinee was achieved by inoculation of this mixture into another chimpanzee. These results suggest that vaccination with a peptide-vaccine of homologous HVR1 is effective in the chimpanzee.
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