[HTML][HTML] Silencing of the gene for the β subunit of human chorionic gonadotropin by the embryonic transcription factor Oct-3/4
L Liu, RM Roberts - Journal of Biological Chemistry, 1996 - ASBMB
The transcription factor Oct-3/4 may be important in maintaining embryonic cells in an
undifferentiated state. It is probably down-regulated at about the time that human chorionic
gonadotropin (hCG) is first expressed in embryonic trophectoderm. Here we report that Oct-
3/4 strongly inhibits the hCGβ subunit (hCGβ) promoter in JAr choriocarcinoma cells. Oct-3/4
reduced chloramphenicol acetyltransferase (CAT) reporter expression from the− 305hCGβ
promoter by about 90% in transient co-transfection assays, but had no effect on expression …
undifferentiated state. It is probably down-regulated at about the time that human chorionic
gonadotropin (hCG) is first expressed in embryonic trophectoderm. Here we report that Oct-
3/4 strongly inhibits the hCGβ subunit (hCGβ) promoter in JAr choriocarcinoma cells. Oct-3/4
reduced chloramphenicol acetyltransferase (CAT) reporter expression from the− 305hCGβ
promoter by about 90% in transient co-transfection assays, but had no effect on expression …