[HTML][HTML] Characterization of rhodopsin mutants that bind transducin but fail to induce GTP nucleotide uptake: classification of mutant pigments by fluorescence …
OP Ernst, KP Hofmann, TP Sakmar - Journal of Biological Chemistry, 1995 - ASBMB
The photoreceptor rhodopsin is a seven-transmembrane helix receptor that activates the G
protein transducin in response to light. Several site-directed rhodopsin mutants have been
reported to be defective in transducin activation. Two of these mutants bound transducin in
response to light, but failed to release the bound transducin in the presence of GTP (Franke,
RR, König, B., Sakmar, TP, Khorana, HG, and Hofmann, KP (1990) Science 250, 123-125).
The present study was carried out to determine the nucleotide-binding state of transducin as …
protein transducin in response to light. Several site-directed rhodopsin mutants have been
reported to be defective in transducin activation. Two of these mutants bound transducin in
response to light, but failed to release the bound transducin in the presence of GTP (Franke,
RR, König, B., Sakmar, TP, Khorana, HG, and Hofmann, KP (1990) Science 250, 123-125).
The present study was carried out to determine the nucleotide-binding state of transducin as …
