A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma
H Volland, P Pradelles, P Ronco, M Azizi… - Journal of …, 1999 - Elsevier
We have developed a new enzyme immunometric assay for angiotensin II (AII) based on
SPIE-IA technology (solid-phase immobilized epitope-immunoassay). A monoclonal
antibody with optimal properties (mAb3 131) was selected from a series of 19 anti-AII mAbs.
The mAb had to be purified from ascitic fluid in a specific manner in order to remove
endogenous AII from the antibody-binding sites. We established a sensitive (minimum
detectable concentration 0.5 pg/ml) and precise (CV below 15% in the 2–100 pg/ml range) …
SPIE-IA technology (solid-phase immobilized epitope-immunoassay). A monoclonal
antibody with optimal properties (mAb3 131) was selected from a series of 19 anti-AII mAbs.
The mAb had to be purified from ascitic fluid in a specific manner in order to remove
endogenous AII from the antibody-binding sites. We established a sensitive (minimum
detectable concentration 0.5 pg/ml) and precise (CV below 15% in the 2–100 pg/ml range) …