Solid phase synthesis of phosphorothioate oligonucleotides utilizing diethyldithiocarbonate disulfide (DDD) as an efficient sulfur transfer reagent
ZS Cheruvallath, RK Kumar, C Rentel… - … and Nucleic Acids, 2003 - Taylor & Francis
ZS Cheruvallath, RK Kumar, C Rentel, DL Cole, VT Ravikumar
Nucleosides, Nucleotides and Nucleic Acids, 2003•Taylor & FrancisDiethyldithiodicarbonate (DDD), a cheap and easily prepared compound, is found to be a
rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate
oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality
based on IP-LC-MS compares well with high quality oligonucleotides synthesized using
phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.
rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate
oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality
based on IP-LC-MS compares well with high quality oligonucleotides synthesized using
phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.
Abstract
Diethyldithiodicarbonate (DDD), a cheap and easily prepared compound, is found to be a rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality based on IP-LC-MS compares well with high quality oligonucleotides synthesized using phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.
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