[42] Legumain: Asparaginyl endopeptidase
SI Ishii - Methods in enzymology, 1994 - Elsevier
Publisher Summary This chapter describes asparaginyl endopeptidase from jack bean (C.
ensiformis). During the characterization and purification of legumain from jack bean seeds,
DNP-Pro-GIu-Ala-Asn-Val-Ile-Arg-NH 2 (DNP, dinitrophenyl) and DNP-Pro-GIu-Ala-Asn-NH
2, which contain a sequence similar to that around one of the processing sites in
proconcanavalin A, are used as substrates. The presence of the DNP group with absorption
near 370 nm allows easy monitoring of separation between an enzymatic reaction product …
ensiformis). During the characterization and purification of legumain from jack bean seeds,
DNP-Pro-GIu-Ala-Asn-Val-Ile-Arg-NH 2 (DNP, dinitrophenyl) and DNP-Pro-GIu-Ala-Asn-NH
2, which contain a sequence similar to that around one of the processing sites in
proconcanavalin A, are used as substrates. The presence of the DNP group with absorption
near 370 nm allows easy monitoring of separation between an enzymatic reaction product …