Superoxide anion generation by human neutrophils exposed to monosodium urate. Effect of protein adsorption and complement activation
S Abramson, ST Hoffstein… - Arthritis & Rheumatism …, 1982 - Wiley Online Library
S Abramson, ST Hoffstein, G Weissmann
Arthritis & Rheumatism: Official Journal of the American College …, 1982•Wiley Online LibraryWe studied the capacities of naked and proteincoated monosodium urate (MSU) crystals to
stimulate superoxide anion (O2 article empty ̄. )releasebyhumanpolymorphonuclearleukoc…
(PMN).UncoatedMSUstimulatedsignificantO2articl… ̄. productionbycytochalasinB‐
treatedPMN.PrecoatingMSUwithIgGcausedanincreasein… ̄. production,whereasprecoatingheatedMSUwithserumor…
̄. release.UnheatedMSUcrystals,whichactivatecomplementtoagreaterextentt…,
alsoprovokedO2articleempty ̄. generation,aneffectagainabrogatedbyprecoatingwithse….CoincubationofunheatedMSUandplasmare …
stimulate superoxide anion (O2 article empty ̄. )releasebyhumanpolymorphonuclearleukoc…
(PMN).UncoatedMSUstimulatedsignificantO2articl… ̄. productionbycytochalasinB‐
treatedPMN.PrecoatingMSUwithIgGcausedanincreasein… ̄. production,whereasprecoatingheatedMSUwithserumor…
̄. release.UnheatedMSUcrystals,whichactivatecomplementtoagreaterextentt…,
alsoprovokedO2articleempty ̄. generation,aneffectagainabrogatedbyprecoatingwithse….CoincubationofunheatedMSUandplasmare …
We studied the capacities of naked and proteincoated monosodium urate (MSU) crystals to stimulate superoxide anion (O2 article empty ̄. )releasebyhumanpolymorphonuclearleukocytes(PMN).UncoatedMSUstimulatedsignificantO2articleempty ̄. productionbycytochalasinB‐treatedPMN.PrecoatingMSUwithIgGcausedanincreaseinmeanO2articleempty ̄. production,whereasprecoatingheatedMSUwithserumorplasmainhibitedO2articleempty ̄. release.UnheatedMSUcrystals,whichactivatecomplementtoagreaterextentthanheatedcrystals,alsoprovokedO2articleempty ̄. generation,aneffectagainabrogatedbyprecoatingwithserumbutnotwithplasma.CoincubationofunheatedMSUandplasmaresultedinanenhancementofO2articleempty ̄. generation.TheresultsoftheseexperimentssupportthehypothesisthatadsorbedproteinsmodulatethephlogisticpotentialofMSUandthatthesurfaceactivationofhumoralmediatorscontributestothelocalinflammatoryresponse.
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