The murine homolog of the interleukin‐8 receptor CXCR‐2 is essential for the occurrence of neutrophilic inflammation in the air pouch model of acute urate crystal …
R Terkeltaub, S Baird, P Sears… - … : Official Journal of …, 1998 - Wiley Online Library
R Terkeltaub, S Baird, P Sears, R Santiago, W Boisvert
Arthritis & Rheumatism: Official Journal of the American College …, 1998•Wiley Online LibraryObjective Acute neutrophil‐dependent inflammation is central to acute gout. Urate crystals
induce different classes of neutrophil chemotaxins, including certain chemokines (eg,
interleukin‐8 [IL‐8], growth‐related oncogene α [GROα]) that share CXCR‐2 as a receptor.
This study was undertaken to assess the role of CXCR‐2 ligands in a model of acute gout.
Methods Urate crystals were injected into subcutaneous air pouches in mice that expressed
or lacked the murine CXCR‐2 homolog (mIL‐8RH), and the development of neutrophilic …
induce different classes of neutrophil chemotaxins, including certain chemokines (eg,
interleukin‐8 [IL‐8], growth‐related oncogene α [GROα]) that share CXCR‐2 as a receptor.
This study was undertaken to assess the role of CXCR‐2 ligands in a model of acute gout.
Methods Urate crystals were injected into subcutaneous air pouches in mice that expressed
or lacked the murine CXCR‐2 homolog (mIL‐8RH), and the development of neutrophilic …
Objective
Acute neutrophil‐dependent inflammation is central to acute gout. Urate crystals induce different classes of neutrophil chemotaxins, including certain chemokines (e.g., interleukin‐8 [IL‐8], growth‐related oncogene α [GROα]) that share CXCR‐2 as a receptor. This study was undertaken to assess the role of CXCR‐2 ligands in a model of acute gout.
Methods
Urate crystals were injected into subcutaneous air pouches in mice that expressed or lacked the murine CXCR‐2 homolog (mIL‐8RH), and the development of neutrophilic inflammation was assessed.
Results
In normal mice, urate crystals induced a 10‐fold increase (P < 0.01) in pouch fluid leukocytes (principally neutrophils) at 4 hours. Leukocytes adhered to the pouch lining, where crystals, the mIL‐8RH ligand KC/GROα and cells bearing mIL‐8RH were abundant. In mIL‐8RH‐/‐ mice, urate crystals induced a proteinaceous leukocyte‐poor exudate at 4 hours, despite crystal‐induced activation of resident cells (documented by KC/GROα expression).
Conclusion
Chemokines that bind the IL‐8 receptor CXCR‐2 are essential for the development of acute neutrophilic inflammation in response to urate crystals in the subcutaneous air pouch model.
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