Activation of the fifth component of human complement (C5) induced by monosodium urate crystals: C5 convertase assembly on the crystal surface
IJ Russell, C Mansen, LM Kolb, WP Kolb - Clinical immunology and …, 1982 - Elsevier
Incubation of monosodium urate crystals (MSUC) with normal human serum (NHS) resulted
in the production of a factor chemotactic for neutrophils which was inactivated by
monospecific antiserum to human C5, indicating it to be a product of C5 activation. When
MSUC were incubated with NHS containing radioiodinated C5, a significant portion of the
label was incorporated into a 1× 106 MW, fluid phase complex identified as SC5b-9. The
complex had a sedimentation velocity of 23.5 S and contained C5b as demonstrated by …
in the production of a factor chemotactic for neutrophils which was inactivated by
monospecific antiserum to human C5, indicating it to be a product of C5 activation. When
MSUC were incubated with NHS containing radioiodinated C5, a significant portion of the
label was incorporated into a 1× 106 MW, fluid phase complex identified as SC5b-9. The
complex had a sedimentation velocity of 23.5 S and contained C5b as demonstrated by …