Activation of the alternative pathway of complement by monosodium urate crystals
TR Fields, SB Abramson, G Weissmann… - Clinical Immunology and …, 1983 - Elsevier
Monosodium urate crystals (MSU) have been shown to activate the alternative pathway of
complement in a dose-and time-dependent fashion at 37° C. Activation was maximal upon
addition of 10–20 mg/ml monosodium urate crystals to C2-deficient human serum (C2D) or
normal human serum containing 5 mM MgEGTA. Immunoelectrophoretic analysis of such
treated sera demonstrated cleavage of C3 and factor B. Incubation of highly purified C3 and
factor B with 10 mg/ml MSU did not, however, affect their immunoelectrophoretic pattern …
complement in a dose-and time-dependent fashion at 37° C. Activation was maximal upon
addition of 10–20 mg/ml monosodium urate crystals to C2-deficient human serum (C2D) or
normal human serum containing 5 mM MgEGTA. Immunoelectrophoretic analysis of such
treated sera demonstrated cleavage of C3 and factor B. Incubation of highly purified C3 and
factor B with 10 mg/ml MSU did not, however, affect their immunoelectrophoretic pattern …