The isolation of crystalline 22R-hydroxycholesterol and 20α, 22R-dihydroxycholesterol from bovine adrenals
R Dixon, T Furutachi, S Lieberman - Biochemical and Biophysical …, 1970 - Elsevier
R Dixon, T Furutachi, S Lieberman
Biochemical and Biophysical Research Communications, 1970•ElsevierUsing tritiated internal standards, crystalline 22R-hydroxycholesterol and 20α, 22R-
dihydroxycholesterol have been isolated for the first time from bovine adrenal glands. The
identity of the sterols was established by comparison of their melting points and infrared
spectra with those of authentic standards. The tissue concentration of the sterols, as
determined by both direct weighing of crystalline samples and the double-isotope derivative
technique, indicated the presence of 1.5 mg/kg of 22R-hydroxycholesterol and 2.15 mg/kg of …
dihydroxycholesterol have been isolated for the first time from bovine adrenal glands. The
identity of the sterols was established by comparison of their melting points and infrared
spectra with those of authentic standards. The tissue concentration of the sterols, as
determined by both direct weighing of crystalline samples and the double-isotope derivative
technique, indicated the presence of 1.5 mg/kg of 22R-hydroxycholesterol and 2.15 mg/kg of …
Abstract
Using tritiated internal standards, crystalline 22R-hydroxycholesterol and 20α,22R-dihydroxycholesterol have been isolated for the first time from bovine adrenal glands. The identity of the sterols was established by comparison of their melting points and infrared spectra with those of authentic standards. The tissue concentration of the sterols, as determined by both direct weighing of crystalline samples and the double-isotope derivative technique, indicated the presence of 1.5 mg/kg of 22R-hydroxycholesterol and 2.15 mg/kg of 20α,22R-dihydroxycholesterol. The presence of neither the monosulfate nor the disulfate of 22R-hydroxycholesterol could be established.
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