Calcium-dependent plasma membrane binding and cell lysis by perforin are mediated through its C2 domain: A critical role for aspartate residues 429, 435, 483, and …
I Voskoboinik, MC Thia, J Fletcher, A Ciccone… - Journal of Biological …, 2005 - ASBMB
The lymphocyte pore-forming protein perforin is essential for maintaining immune
homeostasis and for effective defense against intracellular pathogens. To date, there have
been no reported structure-function studies to substantiate the function of any putative
domains of perforin, which have been postulated totally on primary sequence similarities
with domains in other proteins. In this report, we have used recently developed modalities
for expressing full-length perforin and robust functional assays to investigate one of the …
homeostasis and for effective defense against intracellular pathogens. To date, there have
been no reported structure-function studies to substantiate the function of any putative
domains of perforin, which have been postulated totally on primary sequence similarities
with domains in other proteins. In this report, we have used recently developed modalities
for expressing full-length perforin and robust functional assays to investigate one of the …
