In vivo osmoregulation of Na/myo-inositol cotransporter mRNA in rat kidney medulla.
A Yamauchi, T Nakanishi, Y Takamitsu… - Journal of the …, 1994 - journals.lww.com
Abstract myo-Inositol, a major compatible osmolyte in renal medulla, is accumulated in
kidney-derived epithelial cells cultured in hypertonic media via Na/myoinositol cotransporter
(SMIT). The altered medium osmolality of Madin-Darby canine kidney cells leads to changes
in the transcription of the SMIT gene and mRNA abundance. To investigate whether SMIT is
regulated by tonicity in vivo, renal medullary myoinositol and SMIT mRNA was measured in
rats in hydrated and dehydrated states. Rats were divided into two groups:(1) hydrated rats …
kidney-derived epithelial cells cultured in hypertonic media via Na/myoinositol cotransporter
(SMIT). The altered medium osmolality of Madin-Darby canine kidney cells leads to changes
in the transcription of the SMIT gene and mRNA abundance. To investigate whether SMIT is
regulated by tonicity in vivo, renal medullary myoinositol and SMIT mRNA was measured in
rats in hydrated and dehydrated states. Rats were divided into two groups:(1) hydrated rats …