Chronic infusion of loop diuretics into animals induces structural and functional changes in
the distal nephron. These changes include increases in the activity of the thiazide-sensitive
Na+/Cl--cotransporter (NCC). The NCC was recently demonstrated to be an aldosterone-
induced protein. These experiments were designed to test the hypotheses that chronic loop
diuretic infusion, with replacement of NaCl losses, increases NCC protein abundance and
that this effect results, in part, from stimulation by aldosterone. Sprague-Dawley rats received …