[HTML][HTML] Regulation of basic fibroblast growth factor binding and activity by cell density and heparan sulfate
TP Richardson, V Trinkaus-Randall… - Journal of Biological …, 1999 - ASBMB
The role of cell density in modulating basic fibroblast growth factor binding and activity was
investigated. A primary corneal stromal fibroblast cell culture system was used, since these
cells do not constitutively express heparan sulfate proteoglycansin vivo except after injury. A
3–5-fold reduction in bFGF binding per cell was observed as cell density increased from
1000 to 35,000 cells/cm 2. The cell density-dependent change in bFGF binding was not the
result of altered FGFR expression as determined by equilibrium binding experiments and by …
investigated. A primary corneal stromal fibroblast cell culture system was used, since these
cells do not constitutively express heparan sulfate proteoglycansin vivo except after injury. A
3–5-fold reduction in bFGF binding per cell was observed as cell density increased from
1000 to 35,000 cells/cm 2. The cell density-dependent change in bFGF binding was not the
result of altered FGFR expression as determined by equilibrium binding experiments and by …
