[HTML][HTML] Glucose stimulates the activation domain potential of the PDX-1 homeodomain transcription factor
HV Petersen, M Peshavaria, AA Pedersen, J Philippe… - FEBS letters, 1998 - Elsevier
HV Petersen, M Peshavaria, AA Pedersen, J Philippe, R Stein, OD Madsen, P Serup
FEBS letters, 1998•ElsevierGlucose-stimulated expression of the insulin gene in β cells is mediated by the PDX-1
transcription factor. In this report, we show that stimulation results from effects on activation
and DNA-binding potential. Thus, glucose specifically stimulated expression in MIN6 β cells
from chimeras of PDX-1 and the GAL4 DNA-binding domain which spanned the N-terminal
PDX-1 activation domain located between amino acids 1 to 79. GAL4: PDX activity was
induced over physiological glucose concentrations and was also regulated by effectors of …
transcription factor. In this report, we show that stimulation results from effects on activation
and DNA-binding potential. Thus, glucose specifically stimulated expression in MIN6 β cells
from chimeras of PDX-1 and the GAL4 DNA-binding domain which spanned the N-terminal
PDX-1 activation domain located between amino acids 1 to 79. GAL4: PDX activity was
induced over physiological glucose concentrations and was also regulated by effectors of …
Glucose-stimulated expression of the insulin gene in β cells is mediated by the PDX-1 transcription factor. In this report, we show that stimulation results from effects on activation and DNA-binding potential. Thus, glucose specifically stimulated expression in MIN6 β cells from chimeras of PDX-1 and the GAL4 DNA-binding domain which spanned the N-terminal PDX-1 activation domain located between amino acids 1 to 79. GAL4:PDX activity was induced over physiological glucose concentrations and was also regulated by effectors of this response. The level of endogenous PDX-1 binding and phosphorylation were also induced under these conditions. We discuss how changes in PDX-1 phosphorylation may influence activity in glucose-treated β cells.
Elsevier
