Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles
KD Jacob, KA Eckert - Mutation Research/Fundamental and Molecular …, 2007 - Elsevier
Slipped strand mispairing during DNA synthesis is one proposed mechanism for
microsatellite or short tandem repeat (STR) mutation. However, the DNA polymerase (s)
responsible for STR mutagenesis have not been determined. In this study, we investigated
the effect of the Escherichia colidinB gene product (Pol IV) on mononucleotide and
dinucleotide repeat stability, using an HSV-tk gene episomal reporter system for
microsatellite mutations. For the control vector (HSV-tk gene only) we observed a statistically …
microsatellite or short tandem repeat (STR) mutation. However, the DNA polymerase (s)
responsible for STR mutagenesis have not been determined. In this study, we investigated
the effect of the Escherichia colidinB gene product (Pol IV) on mononucleotide and
dinucleotide repeat stability, using an HSV-tk gene episomal reporter system for
microsatellite mutations. For the control vector (HSV-tk gene only) we observed a statistically …