Allergen specificity of IgG4-expressing B cells in patients with grass pollen allergy undergoing immunotherapy
LK James, H Bowen, RA Calvert, TS Dodev… - Journal of Allergy and …, 2012 - Elsevier
BACKGROUND: Serum IgG4 responses to allergen immunotherapy are well documented as
blocking allergen binding to receptor-bound IgE on antigen-presenting cells and effector
cells, but the molecular characteristics of treatment-induced IgG4, particularly in relation to
expressed antibody, are poorly defined. OBJECTIVES: We aimed to clone and express
recombinant IgG4 from patients receiving grass pollen immunotherapy using single B cells
to obtain matched heavy-and light-chain pairs. METHODS: IgG4+ B cells were enriched from …
blocking allergen binding to receptor-bound IgE on antigen-presenting cells and effector
cells, but the molecular characteristics of treatment-induced IgG4, particularly in relation to
expressed antibody, are poorly defined. OBJECTIVES: We aimed to clone and express
recombinant IgG4 from patients receiving grass pollen immunotherapy using single B cells
to obtain matched heavy-and light-chain pairs. METHODS: IgG4+ B cells were enriched from …
