[HTML][HTML] p90RSK is a serum-stimulated Na+/H+ exchanger isoform-1 kinase: regulatory phosphorylation of serine 703 of Na+/H+ exchanger isoform-1
E Takahashi, J Abe, B Gallis, R Aebersold… - Journal of Biological …, 1999 - ASBMB
The Na+/H+ exchanger isoform-1 (NHE-1) is the key member of a family of exchangers that
regulates intracellular pH and cell volume. Activation of NHE-1 by growth factors is rapid,
correlates with increased NHE-1 phosphorylation and cell alkalinization, and plays a role in
cell cycle progression. By two-dimensional tryptic peptide mapping of immunoprecipitated
NHE-1, we identify serine 703 as the major serum-stimulated amino acid. Mutation of serine
703 to alanine had no effect on acid-stimulated Na+/H+ exchange but completely prevented …
regulates intracellular pH and cell volume. Activation of NHE-1 by growth factors is rapid,
correlates with increased NHE-1 phosphorylation and cell alkalinization, and plays a role in
cell cycle progression. By two-dimensional tryptic peptide mapping of immunoprecipitated
NHE-1, we identify serine 703 as the major serum-stimulated amino acid. Mutation of serine
703 to alanine had no effect on acid-stimulated Na+/H+ exchange but completely prevented …