Bone marrow in patients with pseudarthrosis. A study of progenitor cells by in vitro cloning
P Hernigou, F Beaujean - … chirurgie orthopedique et reparatrice de l' …, 1997 - europepmc.org
P Hernigou, F Beaujean
Revue de chirurgie orthopedique et reparatrice de l'appareil moteur, 1997•europepmc.orgMethods The cell density in the bone marrow in these sites was studied in vitro with material
taken from the non union site. Samples were taken by aspiration with a trocar located by
image intensification. Assays of CFU-GM were chosen to quantify the hematopoietic activity
of the marrow, and of CFU-F to quantify stroma cells activity. Cell densities in the non union
site were compared to those in patients' own iliac crests, and also to iliac crests densities of
a control population of bone marrow donors. Results The bone marrow of non union site …
taken from the non union site. Samples were taken by aspiration with a trocar located by
image intensification. Assays of CFU-GM were chosen to quantify the hematopoietic activity
of the marrow, and of CFU-F to quantify stroma cells activity. Cell densities in the non union
site were compared to those in patients' own iliac crests, and also to iliac crests densities of
a control population of bone marrow donors. Results The bone marrow of non union site …
Methods
The cell density in the bone marrow in these sites was studied in vitro with material taken from the non union site. Samples were taken by aspiration with a trocar located by image intensification. Assays of CFU-GM were chosen to quantify the hematopoietic activity of the marrow, and of CFU-F to quantify stroma cells activity. Cell densities in the non union site were compared to those in patients' own iliac crests, and also to iliac crests densities of a control population of bone marrow donors.
Results
The bone marrow of non union site contained low levels of progenitor cells. Especially surprising was that progenitors were also sparse in the bone marrow of the iliac crest of patients with non union, compared with control bone marrow donors. In several cases, certain general factors are probably responsible for the abnormally low levels of progenitor cells, not only in the non union site, but also in the iliac crest.
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