Insulin release from column-perifused isolated islets of uremic rats

Y Nakamura, T Yoshida, S Kajiyama, Y Kitagawa… - Nephron, 1985 - karger.com
Y Nakamura, T Yoshida, S Kajiyama, Y Kitagawa, T Kanatsuna, M Kondo
Nephron, 1985karger.com
In order to examine the insulin secretion in chronic renal failure, isolated pancreatic islets
either from uremic rats or from control rats were mixed into a short column of Bio-Gel P-2
polyacrylamide beads and perifused. Uremic rats had higher concentrations of blood urea
nitrogen, serum creatinine, and immunoreactive insulin and lower concentration of plasma
1, 25-(OH) 2D3 than control rats. Although the basal insulin release in the presence of 5.0
mMglucose showed no difference between uremic and control rats, the initial insulin release …
Abstract
In order to examine the insulin secretion in chronic renal failure, isolated pancreatic islets either from uremic rats or from control rats were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused. Uremic rats had higher concentrations of blood urea nitrogen, serum creatinine, and immunoreactive insulin and lower concentration of plasma 1,25-(OH)2D3 than control rats. Although the basal insulin release in the presence of 5.0 mMglucose showed no difference between uremic and control rats, the initial insulin release in the presence of 16.2 mMglucose was significantly lower (p < 0.05) in uremic than in controls rats. The insulin content in islets was not different between both groups. These findings suggest that there might be impairment of the initial insulin secretion without changes of insulin content in pancreatic islets in uremia.
Karger