An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow

MB Lutz, N Kukutsch, ALJ Ogilvie, S Rößner… - Journal of …, 1999 - Elsevier
As dendritic cells (DC) are rare populations in all organs, their generation from
hematopoietic precursors in large quantities has proven critical to study their biology. From
murine bone marrow about 5× 106 cells at 70% purity are obtained per mouse after 8 days
of culture with GM-CSF. We have improved this standard method and routinely achieve a 50-
fold higher yield, ie, 1–3× 108 immature and mature DC per mouse at 90–95% purity. The
major modifications were:(i) the avoidance of any active depletion of bone marrow cell …