Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins
CAM Wu, TL Roth, Y Baglaenko, DM Ferri… - Journal of …, 2018 - Elsevier
Genome editing in human cells with targeted nucleases now enables diverse experimental
and therapeutic genome engineering applications, but extension to primary human B cells
remains limited. Here we report a method for targeted genetic engineering in primary human
B cells, utilizing electroporation of CRISPR-Cas9 ribonucleoproteins (RNPs) to introduce
gene knockout mutations at protein-coding loci with high efficiencies that in some cases
exceeded 80%. Further, we demonstrate knock-in editing of targeted nucleotides with …
and therapeutic genome engineering applications, but extension to primary human B cells
remains limited. Here we report a method for targeted genetic engineering in primary human
B cells, utilizing electroporation of CRISPR-Cas9 ribonucleoproteins (RNPs) to introduce
gene knockout mutations at protein-coding loci with high efficiencies that in some cases
exceeded 80%. Further, we demonstrate knock-in editing of targeted nucleotides with …