Peptide: MHC tetramer-based enrichment of epitope-specific T cells
FP Legoux, JJ Moon - JoVE (Journal of Visualized Experiments), 2012 - jove.com
A basic necessity for researchers studying adaptive immunity with in vivo experimental
models is an ability to identify T cells based on their T cell antigen receptor (TCR) specificity.
Many indirect methods are available in which a bulk population of T cells is stimulated in
vitro with a specific antigen and epitope-specific T cells are identified through the
measurement of a functional response such as proliferation, cytokine production, or
expression of activation markers1. However, these methods only identify epitope-specific T …
models is an ability to identify T cells based on their T cell antigen receptor (TCR) specificity.
Many indirect methods are available in which a bulk population of T cells is stimulated in
vitro with a specific antigen and epitope-specific T cells are identified through the
measurement of a functional response such as proliferation, cytokine production, or
expression of activation markers1. However, these methods only identify epitope-specific T …