Measles virus–specific plasma cells are prominent in subacute sclerosing panencephalitis CSF
GP Owens, AM Ritchie, DH Gilden, MP Burgoon… - Neurology, 2007 - AAN Enterprises
GP Owens, AM Ritchie, DH Gilden, MP Burgoon, D Becker, JL Bennett
Neurology, 2007•AAN EnterprisesObjective: To demonstrate the specificity of expanded CD138+ plasma cell clones recovered
from the CSF of a patient with subacute sclerosing panencephalitis (SSPE) for measles virus
(MV). Methods: IgG variable region sequences of single-antibody-secreting CD138+ cells
sorted from SSPE CSF were amplified by single-cell PCR and analyzed. Human IgG1
recombinant antibodies (rAbs) were produced from four expanded CD138+ clones and
assayed for immunoreactivity against MV proteins. Results: Clonal expansion was a …
from the CSF of a patient with subacute sclerosing panencephalitis (SSPE) for measles virus
(MV). Methods: IgG variable region sequences of single-antibody-secreting CD138+ cells
sorted from SSPE CSF were amplified by single-cell PCR and analyzed. Human IgG1
recombinant antibodies (rAbs) were produced from four expanded CD138+ clones and
assayed for immunoreactivity against MV proteins. Results: Clonal expansion was a …
Objective: To demonstrate the specificity of expanded CD138+ plasma cell clones recovered from the CSF of a patient with subacute sclerosing panencephalitis (SSPE) for measles virus (MV).
Methods: IgG variable region sequences of single-antibody-secreting CD138+ cells sorted from SSPE CSF were amplified by single-cell PCR and analyzed. Human IgG1 recombinant antibodies (rAbs) were produced from four expanded CD138+ clones and assayed for immunoreactivity against MV proteins.
Results: Clonal expansion was a prominent feature of the SSPE plasma cell repertoire, and each of the four rAbs assayed was specific for either the MV fusion or the MV nucleocapsid protein.
Conclusions: Expanded plasma cell clones in the CSF of patients with subacute sclerosing panencephalitis produce disease-relevant antibodies. Recombinant antibodies derived from CSF B cells could provide a tool to identify target antigens in idiopathic inflammatory disorders.
American Academy of Neurology