Antigen-experienced T cells have been divided into CD62L⁺ CCR7⁺ central memory (T_CM) and CD62L⁻ CCR7⁻ effector memory (T_EM) cells. Here, we examined coexpression of CD62L and CCR7 in lymphocytic choriomeningitis virus-specific memory CD8 T cells from both lymphoid and nonlymphoid tissues. Three main points emerged: firstly, memory cells frequently expressed a mixed CD62L⁻ CCR7⁺ phenotype that differed from the phenotypes of classical T_EM and T_CM cells; secondly, T_CM cells were not restricted to lymphoid organs but were also present in significant numbers in nonlymphoid tissues; and thirdly, a major shift from a T_CM to T_EM phenotype was found in memory cells that had been stimulated repetitively with antigen.